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Optimized normal serums for blocking. Alternatively, you can try the tips below: No staining. The primary antibody and the secondary antibody are not compatible.
Putting samples on ice will slow down these processes, but protease and phosphatase inhibitor cocktails are also available. If not using a cocktail, PMSF (50 ...
... servers down substantially, making sites less responsive and useful to you. With this in mind we do not secure insensitive, publicly available information such ...
antibodies that have not been stored correctly. ... antibody, centrifuge at 10,000 x g for 20 seconds to pull down solution that is trapped in the threads of the vial, ...
Not only do antibodies to these histone modifications serve as useful reagents ... To test if the antibody pulls down the target of interest you can perform ChIP up ...
No eluted target protein detected. Target protein not expressed in sample used/low level of target protein expression in sample used. Check the expression profile ...
Methanol fixed samples do not require permeabilization. ... Using a pair of forceps lower the cover slip down onto the mounting medium allowing the liquid to ...
Knockout cell lines against specific genes are not always viable ... Can be difficult to distinguish partner proteins pulled down in a complex from off-target binding.
We are not able to guarantee antibodies that have not been stored correctly. ... centrifuge at 10,000 x g for 20 seconds to pull down solution that is trapped in the ...
When this is not the case, it will be noted on the antibody datasheet, and buffers ... may therefore be problematic for immunoprecipitations and pull-down assays.
In general, Abcam does not sell separate kit components, but some popular extra ... down, and decrease the amount of buffers used proportionally, but it may not ...