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Optimized normal serums for blocking. Alternatively, you can try the tips below: No staining. The primary antibody and the secondary antibody are not compatible.
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Putting samples on ice will slow down these processes, but protease and phosphatase inhibitor cocktails are also available. If not using a cocktail, PMSF (50 ...
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Not only do antibodies to these histone modifications serve as useful reagents ... To test if the antibody pulls down the target of interest you can perform ChIP up ...
We are not able to guarantee antibodies that have not been stored correctly. ... centrifuge at 10,000 x g for 20 seconds to pull down solution that is trapped in the ...
No eluted target protein detected. Target protein not expressed in sample used/low level of target protein expression in sample used. Check the expression profile ...
antibodies that have not been stored correctly. ... antibody, centrifuge at 10,000 x g for 20 seconds to pull down solution that is trapped in the threads of the vial, ...
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This building up and tearing down takes place in the face of an apparent paradox. The greatest majority of these biochemical reactions do not take place ...
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The BCA method is not a true end-point method; that is, the final color continues to develop. However ... Dilute the sample until substances no longer interfere.
Could it be an issue of the proteinase inhibitor cocktail? How can the negative control give a signal while the pull down with the same samples does not give a ...
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Enzymes are affected by changes in pH. The most favorable pH value - the point where the enzyme is most active - is known as the optimum pH. This is ...