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Knockout cell lines against specific genes are not always viable ... Can be difficult to distinguish partner proteins pulled down in a complex from off-target binding.
Not only do antibodies to these histone modifications serve as useful reagents ... To test if the antibody pulls down the target of interest you can perform ChIP up ...
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Optimized normal serums for blocking. Alternatively, you can try the tips below: No staining. The primary antibody and the secondary antibody are not compatible.
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No eluted target protein detected. Target protein not expressed in sample used/low level of target protein expression in sample used. Check the expression profile ...
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